Project Description

A/Prof. Nicole Cloonan

University of Auckland, New Zealand

An Introduction to RNA seq

Next generation sequencing & bioinformatics

Tuesday 3 July 2018

Nicole Cloonan is an Associate Professor in Bioinformatics at The University of Auckland. She was previously an ARC Future Fellow at the QIMR Berghofer Medical Research Institute, and an ARC Postdoctoral Fellow at The University of Queensland. Her work is multi-disciplinary in nature, involving computational biology and bioinformatics, biochemistry, cell biology, and molecular biology – all of which she uses to understand the complexity of RNA systems. She’s pretty awesome, you should come to New Zealand and do a PhD with her.

2018 marks the 10th anniversary of the first RNAseq publications, so you would think that it is not a topic that should still require much in the way of an introduction. After giving a brief historical perspective on the technology, analyses, and how they have matured over the last decade, I will argue that not only have many of the fundamentals of analysis not changed but understanding these fundamentals has become even more important in an era of black-box software analysis. Although these fundamentals will be presented with love, care, and support, those with fragile emotional states (such as PhD students at the end of their RNAseq-based study) may still wish to avoid this presentation.