Project Description

Dr Sam Lukowski

 

Institute for Molecular Bioscience, The University of Queensland

Planning single cell experiments and sample suitability

Advances in single-cell sequencing analysis

Tuesday 4 July 2017

Dr Samuel Lukowski is a senior researcher in the Single Cell and Computational Genomics group at the Institute for Molecular Bioscience at the University of Queensland. Sam is an experienced molecular biologist and uses computational techniques to investigate cellular and genetic heterogeneity at the single-cell level. He is lead analyst on a number of single-cell collaborations, and has spearheaded the implementation and use of the high-throughput single-cell RNA-seq platform in Dr. Joseph Powell’s group.
High-throughput single-cell RNAseq is an incredibly powerful tool for assessing cellular heterogeneity, investigating cell lineage specification, and for identifying rare transcripts and cell subtypes. If you are thinking of performing single cell RNAseq assays, the source of your samples and how they are prepared is crucial to the outcome. In this talk, I will discuss the finer points of scRNAseq experimental design; whether the samples you might be considering for scRNAseq will be suitable; and what options are available for you to help answer that burning biological question.