Project Description

A/Prof. Lachlan Coin

Institute for Molecular Bioscience, The University of Queensland

Use of long-read and long-fragment sequencing for assembling complex genomic regions

Advances in single-cell sequencing analysis

Wednesday 5 July 2017

Lachlan completed a Bachelor of Science at ANU, majoring in Mathematics. After several years out of science working in consulting, he went to the Wellcome Trust Sanger Institute to do a PhD in bioinformatics. Lachlan was a research fellow at the School of Public Health, Imperial College London, largely working on methodology for genome-wide association studies. He returned to Australia in 2012 to start a group at the Institute for Molecular Bioscience, University of Queensland where his group works on developing genomics and bioinformatics tools in infectious disease and cancer.
Several new technologies, including nanopore sequencing, optical mapping and linked-read sequencing, allow characterization of extremely long DNA fragments of 100kb or more.   In this talk I will demonstrate how these reads can be used to assemble complex regions of bacterial, plant and human genomes.  I will discuss examples of how ultra-long reads can be used to uncover previously unknown genomic variation, including chromosome end extension; genomic inversions and tandem repeat variation.
Presentation slides are not available from the speaker.