Project Description

Dr Mirana Ramialison


Australian Regenerative Medicine Institute, Monash University

I’ve got my list of differentially expressed genes, now what?

Bioinformatics methods, models and applications to disease

Wednesday 6 July 2016

Dr Ramialison received her Engineering degree from the University of Luminy, France, after which she worked as a database programmer at the ERATO differentiation project in Kyoto. After her PhD in Developmental Genomics at the European Molecular Biology Laboratory in Heidelberg, Germany, she joined the Victor Chang Cardiac Research Institute in Sydney as an EMBO and HFSP post-doctoral fellow. She is now a Group Leader at the Australian Regenerative Medicine Institute, where she leads her Systems Developmental Biology laboratory researching on heart development, evolution and disease. Dr Ramialison is a NHMRC/Heart Foundation Career Development Fellow.

The development of robust automatised pipelines has allowed the standardisation of the processing of high-throughput sequencing datasets. For instance, RNA-sequencing data can now be easily systematically processed from raw reads directly to differentially expressed genes between various conditions. However, the next steps are less obvious. My talk will focus on downstream analysis where no “standard” pipelines are established. Based on collaborative experiences, I will attempt to provide a non-exhaustive overview of different avenues to explore downstream of an RNA-seq experiment, from the traditional pathway analysis towards systems-level approaches.