Understanding transcription with NGS – a biologist’s perspective – Michael Tallack

Erythropoiesis, the development of red blood cells (erythrocytes), requires precise control of erythroid specific gene expression by several intensely studied transcription factors. Among this set of transcription factors is the zinc ‐ finger transcription factor KLF1 (for Kruppel ‐ like factor 1, formerly known as EKLF), a protein that is absolutely necessary for the formation of mature erythrocytes. Studies of KLF1 by a number of in vitro techniques have provided significant insights into its function and the process of erythropoiesis in general.
More recently, we and others have taken advantage of next ‐ gen DNA sequencing (NGS) to study KLF1 function genome ‐ wide by chromatin immunoprecipitation sequencing (ChIP ‐ seq) and expression profiling (mRNA ‐ seq). I will discuss our journey with ChIP ‐ seq and mRNA ‐ seq and how this has led us to a deeper understanding of how KLF1 orchestrates the differentiation or red blood cells.


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