Next-generation sequencing: an overview of technologies and applications – Matthew Tinning

The advent of next generation of sequencing (NGS) technologies has ushered in a new era of genomics. Several NGS technologies are now available generating up to six orders of magnitude more sequence compared to conventional Sanger methods. NGS is now utilised for everything from de novo whole ‐ genome sequencing to targeted re ‐ sequencing of candidate genes and transcriptomics. This presentation will describe fundamental principles and technologies behind current and emerging next ‐ generation sequencing. The different sequencing chemistries and engineering configurations will be outlined, as well as the advantages and disadvantages of different applications on each commercially available NGS platform.


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