Galaxy Australia https://usegalaxy.org.au is a national service designed for the analysis of genome scale data, with an emphasis on high throughput sequencing. Galaxy Australia provides preinstalled bioinformatic tools and public data, such as reference genomes. The Galaxy web interface does not require knowledge of Unix or programming skills, all analyses can be triggered with a mouse click. The interface also means that the service can be accessed from any web-connected device providing users with flexibility and convenience of a virtual laboratory. For new users we offer step-by-step tutorials covering various topics ranging from basic operations in Galaxy to complex analysis, such as RNA-Seq (differential gene expression analysis with high throughput sequencing data), genome assembly and variant calling. After a simple registration process, users get access to an ample amount of storage, compute resources and diverse public datasets. There is no wait time on registration and users can start data analysis immediately after uploading their data. Galaxy provides easy connections to external services for direct data import from public repositories and visualisation of user data on public servers such as UCSC Genome Browser. Raw data, analysis results or chain tool execution (tool workflow) can be shared with other users or made public. Galaxy workflows are one of the most powerful features of the service, allowing users to perform a series of tasks reproducibly on one or many files. Galaxy workflows record not only parameters for tools, but also tool version, providing a reproducible robustness to data analysis and also providing a historical record of the methodology applied to input data. With over 3,000 registered users Galaxy Australia is actively used for research and training. The talk will provide overview of Galaxy Australia and will be of interest for both researchers and educators.