Assembling next-generation data – Torsten Seemann


De novo assembly is the process of reconstructing a genome’s DNA sequence using only a set of much shorter error ‐ prone sequences (reads) sampled from the genome. A good metaphor is to take a newspaper, make twenty photocopies, shred them all, and then try to reconstruct the original newspaper – somewhat challenging. This presentation will discuss the principles of and approaches to de novo assembly of data from the current NGS platforms (Illumina, SOLiD, 454, IonTorrent, PacBio), and briefly cover upcoming technologies. Other practical issues covered will be of computational and memory requirements, limitations of de novo assembly, terminology, file formats, available software, and an example run ‐ through of an assembly using the Velvet software if time permits.

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