Project Description

Dr Martin Smith

Garvan Institute of Medical Research

RNAseq 2.0: a single molecule revolution

Long read bioinformatics and applications

Tuesday 2 July 2019

Martin is the Genomic Technologies Group Leader at the Kinghorn Centre for Clinical Genomics, located at the Garvan Institute of Medical Research in Sydney, Australia. He is a computational biologist from Canada with a background in genomics, microbiology and immunology. His work has focused on genome and transcriptome annotation using comparative genomics and machine learning. He has been using nanopore sequencing since 2014, with a heavy focus on transcriptomic applications.

High-throughput transcriptomic and epigenomic studies have substantiated the prevalence and dynamics of regulatory regions in the human genome, including the surprising diversity and contentious function of long non-coding RNAs. What additional layers of complexity can single-cell and single-molecule sequencing technologies unravel? How will the observation of native molecules in real-time improve our understanding of health and disease? I will describe genomic and computational strategies for functional transcriptome annotation using nanopore sequencing, with emphasis on targeted RNA sequencing, single cell sequencing, epitranscriptomics, and raw signal analysis.